The 1st Edition of UNO Ultimate DC Comics cards was released by Mattel Games in June 2023. Each box contains 224 game cards plus a sealed pack of four holographic foil cards. The game cards are divided into four unique super hero decks: Aquaman, Green Lantern, Superman, and Wonder Woman. Each character deck includes three Enemy Cards, three Event Cards, and two Location Cards. The instruction booklet is printed in English and French,

The full-art foil variants are available for Wild and Character cards. The foil cards have five different levels of rarity indicated by a colored dot in the bottom-right corner of each card: 🟣Ultra Rare, 🔴Super Rare, 🟡Rare, 🔵Uncommon, and 🟢Common. The odds for each tier have not been disclosed.

Mattel distributed a free UNO Ultimate Superman foil card at the San Diego Comic-Con International (SDCC) from July 19–July 23, 2023. The artwork reprints the cover of Superman vol. 3, #28 (April 2014). The image was illustrated by Ed Benes and colored by Alex Sinclair.

A free UNO Ultimate Batman foil card was distributed at GenCon in the Indiana Convention Center from August 3–6, 2023. The artwork reprints the cover of Detective Comics #583 (February 1988). The image was illustrated by Mike Mignola and colored by Anthony Tollin.

Uno Ultimate was designed by Aaron Weil. The DC Comics decks are compatible with Marvel Comics editions allowing for cross-play. Separate add-on packs for Batman and Harley Quinn were released in August 2023. Each add-on pack includes two foil variants.